low fat purified diet lfd  (Huafukang Technology)

Journal: Nutrients

Article Title: Soluble Fiber Inulin Consumption Limits Alterations of the Gut Microbiota and Hepatic Fatty Acid Metabolism Caused by High-Fat Diet

doi: 10.3390/nu13031037

Figure Lengend Snippet: Fatty acid composition of the diets.

Article Snippet: At 6 weeks of age, mice were fed for 11 weeks a purified low-fat diet (LFD; 10% kcal fat, Research Diets Inc., New Brunswick, NJ, USA) or a purified high-fat diet (HFD; 60% kcal fat, Research Diets Inc.).

Techniques:

Journal: Environmental Health Perspectives

Article Title: Metabolic Phenotype of Wild-Type and As3mt -Knockout C57BL/6J Mice Exposed to Inorganic Arsenic: The Role of Dietary Fat and Folate Intake

doi: 10.1289/EHP3951

Figure Lengend Snippet: Study design. Male and female C57BL/6J (WT) and As3mt -knockout (KO) mice were fed a purified low-fat diet (LFD) with adequate folate content ( 2 mg / kg ) and drank deionized water for 4 weeks after weaning. Baseline metabolic characteristics were assessed prior to exposure to inorganic arsenic (iAs) by measuring fasting blood glucose (FBG) and by intraperitoneal glucose tolerance test (IPGTT). Mice were then exposed to 0 or 100 ppb iAs in drinking water and consumed either a low ( 0.2 mg / kg ) or high ( 10 mg / kg ) folate LFD diet for the next 24 weeks. After 24 weeks, all animals were fed a purified high-fat diet (HFD) with the same low and high folate levels and continued iAs exposure for additional 13 weeks, followed by sacrifice and tissue collection. Metabolic phenotyping, including FBG, IPGTT, fasting plasma insulin (FPI) and assessment of body composition by magnetic resonance imaging (MRI), was conducted at Week 6, Week 24, and/or after 8 weeks on the HFD. Spot urine samples and livers for As speciation analysis were collected at Week 6 and/or at sacrifice. a N, number of mice at the beginning of the study.

Article Snippet: Mice consumed a purified low-fat AIN-93G diet (LFD) (17% calories from fat; Envigo Teklad) with adequate folate content ( 2 mg / kg ) and drank deionized water (DIW) for 2 weeks prior to the initial metabolic phenotyping.

Techniques: Knock-Out, Purification, Clinical Proteomics, Magnetic Resonance Imaging

Journal: Environmental Health Perspectives

Article Title: Metabolic Phenotype of Wild-Type and As3mt -Knockout C57BL/6J Mice Exposed to Inorganic Arsenic: The Role of Dietary Fat and Folate Intake

doi: 10.1289/EHP3951

Figure Lengend Snippet: Fasting plasma insulin and homeostasis model assessment–insulin resistance (HOMA-IR) values of male and female wild-type (WT) and As3mt -knockout (KO) mice fed a low (L) or high folate (H) diet and exposed to 0 or 100 ppb inorganic arsenic (iAs) in drinking water, before and after a high-fat diet (HFD), before and after switching from the low-fat diet (LFD) to the HFD: Fasting plasma insulin and HOMA-IR were measured after 24 weeks on a LFD ( A,C ) and after 8 weeks on a HFD ( B, D ). Mean ± SE for n = 13 – 20 ; specific N numbers are found in . p < 0.05 using post hoc Student’s t -test for the following comparisons: S, males vs. females of the same diet, exposure, and genotype; F, low vs. high folate intake animals of the same genotype, exposure, and sex; G, WT vs. KO mice of the same sex, diet, and exposure; and E, 0 vs. 100 ppb iAs-exposed mice of the same sex, genotype, and folate level.

Article Snippet: Mice consumed a purified low-fat AIN-93G diet (LFD) (17% calories from fat; Envigo Teklad) with adequate folate content ( 2 mg / kg ) and drank deionized water (DIW) for 2 weeks prior to the initial metabolic phenotyping.

Techniques: Clinical Proteomics, Knock-Out